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Colour or no colour in the juvenile shell of the black lip pearl oyster, Pinctada margaritifera? ArchiMer
Trinkler, Nolwenn; Le Moullac, Gilles; Cuif, Jean-pierre; Cochennec-laureau, Nathalie; Dauphin, Yannicke.
Pinctada margaritifera mollusc is cultivated in French Polynesia for the production of black pearls. For this study, the colour of juvenile samples (48 days old) was investigated in the visible range spectra (430-700 nm) using spectrophotometry. A first measurement was done with the soft parts still inside the shell (entire animal). Then, the soft parts were removed in order to do a second measurement on the growing edge of the shell. Comparison of the two measurements shows that the estimation of the living animal colour with unaided eye is strongly influenced by the colour of the soft parts. The use of the International Commission on Illumination (ISI) chromaticity diagram shows that at this growth stage, the shells are "white"; i.e. present no...
Tipo: Text Palavras-chave: Juvenile shell; Colour-scoring; Mollusc; Pearl oyster; Pinctada margaritifera.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18883/16449.pdf
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Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease ArchiMer
Trinkler, Nolwenn; Bardeau, Jean-francois; Marin, Frederic; Labonne, Maylis; Jolivet, Aurelie; Crassous, Philippe; Paillard, Christine.
The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be...
Tipo: Text Palavras-chave: Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14065/11301.pdf
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